Colorectal cancer (CRC) is one of the main cancers that threaten life and health, causing a serious social burden. Long term use of NSAIDs can significantly reduce its incidence rate, but it can not be completely prevented and treated. Resection is an early intervention measure that can be combined with chemotherapy and radiation therapy, but…
L-theanine is a source of fresh and unique flavor in tea, and its content affects the quality and price of tea. theanine has various positive effects on human health, such as antioxidant, anxiety relief, anti-tumor, memory improvement, blood vessel improvement, and immune enhancement. Therefore, L-theanine, as a multifunctional food additive, has attracted much attention due…
Curcumin is a polyphenolic compound with good biological activity. It exists in the plant turmeric and has low water solubility. It has great potential as an antioxidant, anti-inflammatory, anti-cancer and anti diabetes drug. Therefore, curcumin is considered a safe and promising drug that can be used to prevent and treat various diseases. Curcumin is mainly…
As an important component of mammalian cell expression system, CHO cells can be genetically engineered to optimize their metabolic pathways, improve the expression efficiency of exogenous proteins, enhance their adaptability and stability, and provide strong support for the research and production of biopharmaceutical products. In the previous issue, we introduced commonly used methods for genetic…
Research background Lutein is a key carotenoid pigment used in plant photosynthesis, and it is also crucial for maintaining human health. For example, lutein has a protective effect on the eyes and also has antioxidant properties, inhibiting the damage of oxygen free radicals to cells. However, due to the abundance of related coding genes, there…
CRISPR interference (CRISPRi) is a system based on CRISPR. This editing allows ribonucleoprotein (RNP) complexes to be directed and bind to target DNA, interfering with RNA polymerase (RNAP) to inhibit transcription initiation or elongation. Unlike the CRISPR/Cas9 system, CRISPRi allows for a reduction in gene expression by expressing dCas9 or sgRNA under inducible promoters, thereby…
Glucosamine (GlcN) and N-acetylglucosamine (GlcNAc) are important precursor substances for the synthesis of glycosaminoglycans, with extensive application value in many fields. Microbial fermentation has many advantages in the production of GlcN and GlcNAc, and is increasingly favored. This study constructed genetically engineered strains of Escherichia coli with high production of GlcN and GlcNAc, laying the…
Research background: The biosynthesis of natural products usually requires the binding of eukaryotic cytochrome P450 (P450) to P450 reductase, which is physically close and undergoes electron transfer reactions. Research question: Unfortunately, the functional expression of eukaryotic p450 in bacteria is often still difficult. Research content and results: 1) The author of this article artificially approached…
Theodor Escherich, an outstanding physician and scientist of the 19th century, was one of the earliest scientists to explore microorganisms. He founded the field of intestinal pathology and associated his name with the name Escherichia coli, which was discovered and named in 1885. From then on, the “E.coli clan” was endowed with the fate of…
In 2011, countries such as the United States, China, the United Kingdom, Singapore, and Australia launched the “Sc2.0 Project” to redesign and synthesize all 16 chromosomes of brewing yeast. This was the first attempt by humans to design and synthesize the genome of eukaryotes from scratch. On November 8, 2023, the latest research findings…