Characterization and synthetic biology strategies of GMPS

Using engineering bacteria to titrate hydrogen sulfide and induce concentration dependent effects on the host in the gut microbiota

Abstract Hydrogen sulfide (H2S) is a gaseous microbial metabolite, and its role in intestinal diseases is highly controversial. This is mainly due to the difficulty of accurately measuring H2S and the use of model systems that cannot accurately represent the human intestinal environment for research. In this paper, the engineered Escherichia coli designed by the…

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Tetrahydropyrimidine biosynthesis pathway

The titer reaches 115.87 g/L, and under low salt conditions, the engineered glutamic acid rod bacterium produces tetrahydropyrimidine, which is the highest level among this strain to date

Many microorganisms accumulate compatible solutes to counteract external pressures such as high osmotic pressure, high temperature, drying, and freezing. Among numerous compatible solutes, tetrahydropyrimidine stands out for its significant efficacy in effectively protecting halophilic cells from osmotic pressure imbalance and maintaining the stability of intracellular biomolecules. Recent studies have shown that tetrahydropyrimidine has the potential…

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PNAS: A Simple and Robust Experimental Process for Protein Engineering

According to a new study by researchers at the University of Michigan, a protein engineering method using simple, cost-effective experiments and machine learning models can predict which proteins are effective for specific purposes. This method has profound potential in assembling proteins and peptides, and can be used for applications ranging from industrial tools to therapeutic…

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Cell: Cas9 can cause chromosome loss. The Nobel Prize team has developed an improvement plan to enhance the clinical safety of gene editing

The emergence of CRISPR-Cas9 gene editing technology has significantly changed the landscape of gene editing, providing scientists with unprecedented convenience and efficiency in freely manipulating genes. However, the accuracy of CRISPR gene editing is crucial for ensuring clinical safety and avoiding accidental and permanent genotoxicity. In recent years, CRISPR-Cas9 gene editing has made advanced T…

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《Cell》breaks the world record for yeast! The Miracle of Artificial Life

Despite containing only 7.5 artificial chromosomes, highly edited strains still survive and replicate. Biologists have developed a yeast whose genome contains over 50% of artificially synthesized DNA. Standard brewing yeast (Saccharomyces cerevisiae) stores its genetic blueprint on 16 chromosomes. In the new strain, 6.5 of these chromosomes were edited and synthesized in the laboratory, while…

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Nature Review | Academician Sang Yup Lee: Microorganisms as Green Factories for Food and Cosmetics Production

With the increasing awareness of environmental issues and sustainability, the demand for products such as environmental protection, high-quality food, and cosmetics is also increasing. At this critical moment, microorganisms become potential key players, providing important solutions for these seemingly completely different problems. Microbial Cell Factory: A Wonderful Revolution   The field of systemic metabolic engineering…

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