Troubleshooting Low Knockout Efficiency in CRISPR Experiments
The gene editing field underwent a major transformation with CRISPR-Cas9 because it allows scientists to make precise and efficient genomic modifications. The broad application of CRISPR-Cas9 technology does not prevent researchers from facing difficulties particularly related to achieving high knockout efficiency. The most prevalent problem in CRISPR-Cas9 experiments is inadequate gene deletion levels that produce…