CD Biosynsis has successfully established a variety of gene expression systems and is committed to promoting research related to viral structural proteins using in vitro expression systems to express a variety of viral nucleocapsid proteins.
The nucleocapsid protein is the main protein of the viral capsid, which has nucleoprotein properties, binds to nucleic acids, protects them, and plays an important role in the process of virus proliferation and infection. Currently, there are various methods to study the function of viral proteins, among which, in vitro expression of viral structural proteins followed by functional studies is one of the most common means. Based on synthetic biology techniques, viral recombinant structural proteins are obtained by prokaryotic or eukaryotic expression systems and can be used for activity analysis, screening and characterization of viral mutants, and study of virus-host cell interaction mechanisms. Therefore, as the main structural protein of viruses, the large-scale production of nucleocapsid proteins through synthetic biology is of great importance for the study of many viruses.
Figure 1. Viral nucleocapsid proteins (NCs) function in several stages of the virus life cycle. (Ding B, et al., 2016)
Based on the synthetic biology platform, our scientists are dedicated to exploring the application of genetic engineering, protein engineering, and other technologies in the process of nucleocapsid protein production.
Production of Nucleocapsid Protein
We have successfully established a variety of gene expression systems for the production of nucleocapsid proteins. Depending on the expression host, they can be classified as E. coli expression systems, yeast expression systems, mammalian expression systems, and plant expression systems. Based on the above in vitro expression systems, we have realized the production of various viral nucleocapsid proteins. If you would like to synthesize nucleocapsid proteins of other viruses by synthetic biology methods, please contact us directly.
|SARS-CoV-2 nucleocapsid||Ebola virus nucleocapsid||Henipavirus nucleocapsid|
|Retrovirus nucleocapsid||HIV-1 nucleocapsid||Human parainfluenza virus types 1 nucleocapsid|
|Porcine circovirus type 2 nucleocapsid||Akabane virus nucleocapsid||Newcastle disease virus nucleocapsid|
|Rabies virus nucleocapsid||Schmallenberg virus nucleocapsid||Porcine reproductive and respiratory syndrome nucleocapsid|
Optimization of In Vitro Expression of Nucleocapsid Protein
Nucleocapsid proteins are expressed at low levels in vitro, are weakly immunogenic, and do not produce an effective, durable, and widespread cytosolic humoral immune response in the secondary organism. We help our customers to optimize the in vitro expression of nucleocapsid proteins through the following services.
CD Biosynsis can develop tailored tools and customized approaches to harness the power of synthetic biology to drive nucleocapsid protein production and meet the needs of customers in a variety of industries.
CD Biosynsis provides the most comprehensive and efficient solutions for synthetic biology workflows. We are committed to helping our customers solve all problems encountered in nucleocapsid protein production to advance their applications in a wide range of fields. Each of our deliverables will undergo a rigorous quality inspection test to ensure the reliability and accuracy of the results. If you are interested in our services or have any further questions, please do not hesitate to contact us.