CD Biosynsis is committed to developing synthetic biology tools and enabling technologies to help our customers advance the biosynthesis of butanetriol in a more efficient, environmentally friendly, sustainable, and cost-effective manner. Our scientists specializing in synthetic biology chassis development and metabolic engineering are able to identify the suitable host organism and construct optimized synthetic pathways to achieve high yield production of butanetriol.
Butanetriol (BT or BTO) with the molecular formula C4H10O3 is a high-value four-carbon polyol with a variety of industrial applications. Since to date no naturally biosynthetic pathway for BT have been reported, chemical synthesis is the traditional strategy for BT production. However, the chemical synthesis of BT often involves hydrogenation reactions that require harsh reaction conditions (e.g., high temperatures) and the use of expensive metal catalysts. This process requires the use of petroleum-derived substrates and may produce toxic by-products, thus having a negative impact on the environment. Therefore, BT production needs to move towards environmentally friendly technologies using sustainable resources. Bio-based approaches are becoming attractive alternative routes. Advances in synthetic biology have accelerated the metabolic engineering, driving BT biosynthesis green manufacturing.
Figure 1. BTO biosynthesis from D-xylose, L-arabinose and malate as feedstock (A) in Escherichia coli and (B) in Arabidopsis thaliana. (Ghosh D., 2017))
Leveraging our powerful synthetic biology platform, CD Biosynsis can provide our customers with custom synthetic biology services to help them develop effective strategies to achieve efficient biosynthesis of butanetriol.
CD Biosynsis can help our customers overcome challenges in the efficient biosynthesis of butanetriol, such as inefficient enzymes, the lack of suitable synthetic biology chassis and efficient biosynthetic pathways, and the use of expensive raw materials. Our experts are capable of identifying the bottlenecks in the inefficient synthesis of BT and developing efficient synthetic pathways to improve BT production.
Development of Synthetic Biology Chassis for BT Production
We can identify and engineer a suitable host organism as synthetic biology chassis for improved BT production. These organisms can be bacteria, fungi, algae, and plants listed in the table below. If you are interested in other species, please fill out the online inquiry form and tell us more about your project.
|Escherichia coli BL21||Escherichia coli BW25113||Saccharomyces cerevisiae||Chlamydomonas reinhardtii|
|Synechococcus sp. PCC 6803||Synechococcus sp. PCC 7002||Synechococcus elongatus PCC 7942||Arabidopsis thaliana|
Design, Construction, and Optimization of BT Synthetic Pathway
With the aid of computational tools, we can select stepwise chemical intermediates and enzyme candidates for BT biosynthesis and outline candidate BT biosynthetic pathways. We focus on screening the key enzymes of the BT biosynthetic pathway using xylose, arabinose and malate as substrates, such as d-xylose dehydrogenase (XDG), d-xylonate dehydratase (XDT), benzylformate decarboxylase (BFD), aldehyde reductase (AdhP), d-arabinose dehydrogenase (AraDH), d-arabinonate dehydratase (AraD), and 2-keto acid decarboxylase (MdlC). Our expertise in enzyme engineering allows us to help our customers achieve improved BT production by systematical fine-tuning the enzyme expression level and activity.
CD Biosynsis provides customized solutions for synthetic biology-driven butanetriol production to meet the needs of a wide range of industries, including but not limited to medicine, cosmetics, agriculture, polymer materials, pulp and paper industry, and tobacco industry.
As a rapidly growing synthetic biology company, CD Biosynsis is committed to helping our customers meet the growing and evolving demand for bio-based chemical production. All of our deliverables will undergo a rigorous quality testing process to ensure the quality and reliability and can be delivered on time. If you are interested in our services or have any further questions, please do not hesitate to contact us.