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Research background: The biosynthesis of natural products usually requires the binding of eukaryotic cytochrome P450 (P450) to P450 reductase, which is physically close and undergoes electron transfer reactions.<\/p>\n
Research question: Unfortunately, the functional expression of eukaryotic p450 in bacteria is often still difficult.<\/p>\n
Research content and results: 1) The author of this article artificially approached P450-CPR and achieved electron transfer by using the scaffold protein CipB derived from luminescent bacteria; 2) Detect the effectiveness of CipB through the increase or decrease in the production of various metabolites; 3) The construction of the heme pathway and optimization of the culture medium improve production efficiency.<\/p>\n
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Point of interest: The same vector contains either cipA scaffold protein or CiP B scaffold protein, and there is no fusion of CPR and p450 (the content provided in the article is very inconsistent, good, but not easy to read in detail)<\/p>\n
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